| |
|
|
| Registros recuperados : 3 | |
| Registros recuperados : 3 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
27/01/2023 |
|
Data da última atualização: |
24/03/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
MACHADO-ROSA, T. A.; BARBOSA, E. T.; CÔRTES, M. V. de C. B.; LOBO JUNIOR, M. |
|
Afiliação: |
TARIANE A. MACHADO-ROSA, UNIVERSIDADE FEDERAL DE GOIÁS; ELDER TADEU BARBOSA, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MURILLO LOBO JUNIOR, CNPAF. |
|
Título: |
Microtiter method for quantitative assay of IAA from fungal isolates, demonstrated with Trichoderma. |
|
Ano de publicação: |
2023 |
|
Fonte/Imprenta: |
Rhizosphere, v. 25, 100666, 2023. |
|
ISSN: |
2452-2198 |
|
DOI: |
https://doi.org/10.1016/j.rhisph.2023.100666 |
|
Idioma: |
Inglês |
|
Conteúdo: |
A quantitative and accurate method is needed to estimate indole-3-acetic acid (IAA) production by Trichoderma to compare strains and allow the repeatability of experiments in plant-growth promotion studies. Suspensions of 1.0 × 106 conidia. mL-1 of 98 Brazilian strains were grown in potato + dextrose broth in the absence or presence of L-tryptophan (100 mg L-1) in 1 mL microtubes. After 12,000 rpm spinning for 15 min, 150 μL of the super-natant were added to 100 μL of Salkowski reagent with 30 min incubation at room temperature in the dark, with IAA yield spectrophotometrically estimated at 540 nm. Isolates BRM-29474 and BRM-29574 that outperformed in the IAA production increased tomato root and shoot mass and number of root tips in a greenhouse test, endorsing their potential for plant growth promotion. This adjustment allows a quantitative method to estimate an IAA production by Trichoderma spp., which is more informative than the standard qualitative method. |
|
Palavras-Chave: |
Biocontrol; Plant growth promotion; Stepwise screening. |
|
Thesagro: |
Estimulante de Crescimento Vegetal; Fungo; Trichoderma. |
|
Thesaurus NAL: |
Auxins; Plant growth; Root exudates. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 01851naa a2200289 a 4500
001 2151274
005 2023-03-24
008 2023 bl uuuu u00u1 u #d
022 $a2452-2198
024 7 $ahttps://doi.org/10.1016/j.rhisph.2023.100666$2DOI
100 1 $aMACHADO-ROSA, T. A.
245 $aMicrotiter method for quantitative assay of IAA from fungal isolates, demonstrated with Trichoderma.$h[electronic resource]
260 $c2023
520 $aA quantitative and accurate method is needed to estimate indole-3-acetic acid (IAA) production by Trichoderma to compare strains and allow the repeatability of experiments in plant-growth promotion studies. Suspensions of 1.0 × 106 conidia. mL-1 of 98 Brazilian strains were grown in potato + dextrose broth in the absence or presence of L-tryptophan (100 mg L-1) in 1 mL microtubes. After 12,000 rpm spinning for 15 min, 150 μL of the super-natant were added to 100 μL of Salkowski reagent with 30 min incubation at room temperature in the dark, with IAA yield spectrophotometrically estimated at 540 nm. Isolates BRM-29474 and BRM-29574 that outperformed in the IAA production increased tomato root and shoot mass and number of root tips in a greenhouse test, endorsing their potential for plant growth promotion. This adjustment allows a quantitative method to estimate an IAA production by Trichoderma spp., which is more informative than the standard qualitative method.
650 $aAuxins
650 $aPlant growth
650 $aRoot exudates
650 $aEstimulante de Crescimento Vegetal
650 $aFungo
650 $aTrichoderma
653 $aBiocontrol
653 $aPlant growth promotion
653 $aStepwise screening
700 1 $aBARBOSA, E. T.
700 1 $aCÔRTES, M. V. de C. B.
700 1 $aLOBO JUNIOR, M.
773 $tRhizosphere$gv. 25, 100666, 2023.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
